I am getting different taxaplot . First of all I performed taxonomy analysis with customized database. After that analysis, I added some more sequence and performed taxonomy analysis again, but that taxaplot result is different from first one.
When I did first time sample 2 has moxella species (82%), but in second time sample 2 has Acinetobacter indicus (50%). In second time sample 7 has Streptococcus;s__pneumonia (94%) whereas in first time k__Bacteria was only seen.
As per my culture report, sample 2 has moxella species, sample 7 has streptococcus pneumonia.
I tried many times performing taxanomy analysis. If I get moxella species in sample 2 mean, sample 7 has nothing in taxplot. If I get strptococcus penumonia in sample 7 mean, moxella species was not seen in my taxaplot.
If I add any new sequence mean, that taxaplot look different from previous one. My biggest surprise is I didnt include pseudomonas aeuroginosa sequence in my taxonomy file first time. But that species was seen in my taxplot.
Could anyone tell me why the taxanomy analysis result is coming like this? Herewith, I have attached taxaplot snapshot for your reference.
First time taxonomy analysis result
Second time taxonomy analysis result