Thanks for sharing those details! Jumping in for @thermokarst on this one to help you troubleshoot a bit further.
The error in the screenshot you provided states the following:
Metadata filepath doesn't exist, or the path points to something other than a file.
Please check that the path exists, has read permissions, and points to a regular file (not a directory):
These recommendations are what I would start with (if you haven't already). Double check that your filepath is correct, that you have read permissions on it, and that it is pointing to a file vs. a directory (which it looks like it is, from the above error message).
Additionally, I want to point out the rest of the message just to cover all of your bases.
There may be more errors present in the metadata file.
To get a full report, sample/feature metadata files can be validated with Keemei:
Find details on QIIME 2 metadata requirements here:
These resources will be beneficial if you run into any issues with the formatting in your metadata file.
Hope this helps!