Hi, I have been attempting to run this command:
qiime metadata tabulate \
--m-input-file ./taxonomy.qza \
However, every time I do it will give me an error saying my --m-input-file is not a metadata file. I'm unsure why this is happen as I am following the instructions listed in the Parkinson's Mouse Tutorial. Any help with this would be greatly appreciated. Thank you!
We need some additional information in order to effectively help you:
- What version of QIIME 2 are you using?
- Please copy-and-paste the entire error message, from top to bottom - this is useful context for us to have in trying to help understand the root cause of any errors.
Hi @thermokarst ,
Thank you for your help with this! The version of QIIME 2 I am using is 2021.4.0 and here is the entire error message:
Thanks for sharing those details! Jumping in for @thermokarst on this one to help you troubleshoot a bit further.
The error in the screenshot you provided states the following:
Metadata filepath doesn't exist, or the path points to something other than a file.
Please check that the path exists, has read permissions, and points to a regular file (not a directory):
These recommendations are what I would start with (if you haven't already). Double check that your filepath is correct, that you have read permissions on it, and that it is pointing to a file vs. a directory (which it looks like it is, from the above error message).
Additionally, I want to point out the rest of the message just to cover all of your bases.
There may be more errors present in the metadata file.
To get a full report, sample/feature metadata files can be validated with Keemei:
Find details on QIIME 2 metadata requirements here:
These resources will be beneficial if you run into any issues with the formatting in your metadata file.
Hope this helps!
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