Dear all,
I am really new to QIIME (2) and I was wondering if anyone is able to help me with the taxonomic assignation of my sequences.
I would like to have a taxonomic assigning of my feature ID’s (OTU’s) to the sequences. I was able to create a OTU-table and to get a fasta-file with all the feature ID’s with corresponding sequences. The problem now is that it would take me forever to BLAST every single feature ID to NCBI, so I think it is actually possible to have this done by QIIME 2.
I am totally aware that a taxonomic classifier is probably needed, but I am really not sure how to get such a classifier for COI-sequences (for arthropods to be exact). I was actually able to download a COI training set which has been used in earlier studies, which I think I can use for my sequences right now. The training set regards a tar.gz file. I am just not sure how to proceed from here. Is it even possible to use this training set? How can I import this training set?
Again, I am very new to QIIME, so I am very sorry about being not that specific or being too vague. Please let me know If you have any questions.
Thanks in advance!
Margreet