Dear Antonio,
Thank you very much that helps. I did not realize that deblur had chimera checking built in, the way I read paper is that it used vsearch separately.
I imagine that the newest feature, Analyzing paired end reads in QIIME 2, would likely be the workflow that I use now to import my sequences? My raw data from the sequencer gives me a folder with the R1 and R2 sequences in there, but barcodes are not present. Can I still just follow that protocol and upload that folder or do I need to make any modifications or changes?
Thank you very much for your time and help. I appreciate your guidance and patience with a new user as well.
