# Some troubles with data importation
**URL:** https://forum.qiime2.org/t/some-troubles-with-data-importation/2305
**Category:** User Support
**Tags:** demux, barcode, import
**Created:** [December 21, 2017, 12:55pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305 "2017-12-21T12:55:56Z")
**Posts on this page:** 6
**Page:** 1
### Author:  [@zhenhaoluo](https://forum.qiime2.org/u/zhenhaoluo)
#### Post date: [December 21, 2017, 12:55pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/1 "2017-12-21T12:55:56Z")
Hi,
Hello, there's some troubles with my data importation. There are about more than 100 samples in my dataset, and I have sequenced them in two run, which some of the samples have the same barcode, e.g. Sample 1 in Run I may have the barcode 67 while the Sample 100 in Run II may have the sample barcode 67. And now I would like to analyze them in one dataset. How could I import the data? Which kind of data format should I use?
Thank you!
Zhenhao Luo
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### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [December 22, 2017, 7:30pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/5 "2017-12-22T19:30:42Z")
Hi @zhenhaoluo!
Are your samples already demultiplexed? If so, then the barcodes don't matter -- that is only for demultiplexing multiplexed reads. One thing to keep in mind though is if you plan on running your reads through q2-dada2, you will want to import both of these runs individually, because DADA2's error model assumes that reads are processed on a per-run basis. See the [FMT Tutorial](https://docs.qiime2.org/2017.12/tutorials/fmt/) for an example of processing multiple runs in QIIME 2.
If your data _isn't_ demultiplexed, you might be in a difficult place - QIIME 2 isn't able to account for runs as part of the demultiplexing process, so you would need to find a tool that is capable of doing that (I personally don't know of anything that can handle these kinds of data), or writing out a custom script.
Keep us posted! 🦖
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### Author:  [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [December 22, 2017, 7:39pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/7 "2017-12-22T19:39:23Z")
> [@thermokarst](#):
>
> If your data isn’t demultiplexed, you might be in a difficult place - QIIME 2 isn’t able to account for runs as part of the demultiplexing process, so you would need to find a tool that is capable of doing that (I personally don’t know of anything that can handle these kinds of data), or writing out a custom script.
I think @zhenhaoluo would be able to just import and demultiplex each run separately. Then the overlapping barcodes don't matter, and the feature-tables can be merged later on downstream (like in the FMT tutorial).
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### Author:  [@zhenhaoluo](https://forum.qiime2.org/u/zhenhaoluo)
#### Post date: [December 27, 2017, 3:17pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/8 "2017-12-27T15:17:51Z")
Hi thermokarst!
I may try to do as ebolyen said, Thanks very much~
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### Author:  [@zhenhaoluo](https://forum.qiime2.org/u/zhenhaoluo)
#### Post date: [December 27, 2017, 3:17pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/9 "2017-12-27T15:17:52Z")
Hi ebolyen!
Thank you and I will try to do something as you said~
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [January 27, 2018, 9:18pm UTC](https://forum.qiime2.org/t/some-troubles-with-data-importation/2305/10 "2018-01-27T21:18:03Z")
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