# some questions about cutadapt demux-paired

**URL:** <https://forum.qiime2.org/t/some-questions-about-cutadapt-demux-paired/19223>\
**Category:** User Support\
**Created:** [April 15, 2021, 7:13am UTC](https://forum.qiime2.org/t/some-questions-about-cutadapt-demux-paired/19223 "2021-04-15T07:13:49Z")\
**Posts on this page:** 1\
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**Author:** ![LiuZjiia](https://forum.qiime2.org/user_avatar/forum.qiime2.org/liuzjiia/32/10816_2.png) [@LiuZjiia](https://forum.qiime2.org/u/LiuZjiia)\
**Post date:** [April 15, 2021, 8:58am UTC](https://forum.qiime2.org/t/some-questions-about-cutadapt-demux-paired/19223/3 "2021-04-15T08:58:56Z")

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Thanks for replying, the data comes from my senior, so it has been through a long time. As I know, the data was sequenced by Illumina MiSeq. Actually I tried improve error rate to 0.2, and the I get data indeed, but I'm afraid this way may influence subsequent analysis, and here is my new result.(Plus: another two pics are the result of run demux summary command after getting qza file wen set the error rate 0.1 and 0.2 respectively)

 ![图片](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/b/b7505d67c6e92f9b13fb31c85a60d602ea0ce6f8.png) ![图片](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/7/753caad28d9025e8680ca97b5e63ff4e31f15b1e.png) ![图片](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/8/88dd60063995aa204074e55dd76e9eb515654382.png)

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