Thanks for replying, the data comes from my senior, so it has been through a long time. As I know, the data was sequenced by Illumina MiSeq. Actually I tried improve error rate to 0.2, and the I get data indeed, but I'm afraid this way may influence subsequent analysis, and here is my new result.(Plus: another two pics are the result of run demux summary command after getting qza file wen set the error rate 0.1 and 0.2 respectively)
