Silva classifier training

[Benedict (Benedict Tan)]

Hi,

I would like to classify my query sequences using pre-trained silva classifier contributed by qiime2 society, however it was incompatible with the current qiime2 release (2020.6). Thus, I planned to train my own classifier using the pre-formatted SILVA reference database and taxonomy files (138 release) from the data resource page Data resources — QIIME 2 2020.6.0 documentation. However, the job was killed due to incompatible version of scikit. May I know which version of scikit was used to process and produce the pre-formatted SILVA 138 release? Else, do you have any idea on which version of qiime2 is compatible with the mentioned pre-formatted silva database?

Apart from this, my taxonomic classification using pre-trained classifier always being killed too due to memory error. May I seek the advice on computer core (mine is 6) and memory size (mine is12GB RAM) for the successful classification and classifier training based on your previous experience?

Thank you.

-Benedict-

[SoilRotifer (Mike Robeson)]

Hi @Benedict,

Unless your environment was altered, the current SILVA 138 classifiers should work with 2020.6, as they were made with that version. Do you know which version of scikit-learn you have?

We have an "escape hatch" of sorts... You can simply train the classifier yourself using these files as input to feature-classifier classify-sklearn.

-Mike