I am trying to replicate the analyzes precisely because the materials and methods provided lack several details, to quote a movie: "i feel i was denied critical need-to-know information!"
I have the same .fastq they worked on, so I'm trying to find some results compatible with theirs.
Can the use of nà ive bayes classifier vs vsearch vs blast justify a difference in the percentages of over 40%?
At the moment, unfortunately, I work with 32 gb of ram and following the instructions in the following guide, the procedure is killed after a couple of minutes, I guess for lack of ram.
I tried to download the pre trained classifier from this post, but it turns out to be too old of scikit to work on my qiime version.
Do I have to wait for my supervisor to provide the money to do a hardware upgrade and train the database myself, or is there any working pretrained silva 16S?
