I am working on 16S gut microbiome data. I have received paired-end demultiplexed sequences from the sequencing service provider. I would like to look into the sequence quality before working on dada2. Could you please suggest a pipeline something similar to demux as mentioned in Moving pictures tutorial.
Once you import your sequenced into qiime2 using the manifest format for paired-end reads, the rest of the pipeline is similar to what you were following in the Moving pictures tutorial. For example,
qiime demux summarize
Will still give you the quality plots you are looking for in this case.
Thank you for the reply. I have around 75 samples. I have generated qzv file. However, just wondering if can I view the quality of all the sequences in a single graph.
I have figured it out. Thank you so much for the help
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.