Sure. I will try that. In my post here Lots of unassigned and host DNA in my samples - #2 by Nicholas_Bokulich, we were able to recognize that what I am seeing is probably host DNA not microbiome. Do you think that trying with demultiplexed sequences from the sequencing center might change the results in terms of the host DNA problem? I have heard there might be a bioinformatic method to reduce the sequencing depth to maybe ratify the issue of having too much host DNA instead of re-sequencing. Do you know anything about that and do you think that might have anything to do with my demultiplexing problem here?