# Seqs.fna is not a(n) QIIME1DemuxFormat file
**URL:** https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450
**Category:** User Support
**Tags:** import
**Created:** [January 7, 2018, 6:49pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450 "2018-01-07T18:49:40Z")
**Posts on this page:** 13
**Page:** 1
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 7, 2018, 6:49pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/1 "2018-01-07T18:49:40Z")
Hello Everyone,
I tried importing the output generated from split\_library in qiime1 using the command:
qiime tools import
--input-path seqs.fna
--output-path seqs.qza
--type SampleData[Sequences]
I got a message that it is not a Demuxformat file? Pls. let me know how I can solve this. Thank you very much.
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 8, 2018, 5:26pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/3 "2018-01-08T17:26:40Z")
Hi @Pseudomonas84,
I think the issue is that you are missing the following parameter from your command:
`--source-format QIIME1DemuxFormat`
Could you give that a try? Thanks!
---
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 8, 2018, 7:36pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/5 "2018-01-08T19:36:06Z")
Hello Nicholas,
I tried putting the source format QIIME1DemuxFormat, it still came back with a message that seqs.fna is not a QIIME1DemuxFormat file, is there another way to import this file to qiime2?
Thanks,
Raya
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 8, 2018, 7:42pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/6 "2018-01-08T19:42:06Z")
Hi @Pseudomonas84,
Thanks for attempting to specify the source format. Could you please run the following command (on the unimported data) and share the output:
`head seqs.fna`
`tail seqs.fna`
I want to inspect the first/last several lines to make sure this is not a formatting issue.
Thanks!
---
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 8, 2018, 7:45pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/7 "2018-01-08T19:45:56Z")
heads seqs.fna
> KDC\_1 D00420 org\_bc=GAGATGAC new\_bc=GAGATGAC bc\_diffs=0
> TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCgcgcgTAGGTGGTTTGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTGTGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACCAACACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCcc
> KDC\_10000 D00420 org\_bc=GAGATGAC new\_bc=GAGATGAC bc\_diffs=0
> TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTtttAAGTCCGCCGTCAAATCCCAGGGCTCAACTCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCGTAGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAgagaCGAAAGCTAGGGGAGCGAATGGGATTAGATACCCG
> KDC\_10002 D00420 org\_bc=GAGATGAC new\_bc=GAGATGAC bc\_diffs=0
> TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCAAAACTGACTGACTAGAGTGTGGTAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGACCAACACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCG
> KDC\_10012 D00420 org\_bc=GAGATGAC new\_bc=GAGATGAC bc\_diffs=0
> TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTTAAGTCAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGAAACTGGCAGGCTTGAGTCTTGTAGAGGggggTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCcccTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCG
> KDC\_1002 D00420 org\_bc=GAGATGAC new\_bc=GAGATGAC bc\_diffs=0
> TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTTAAGTCAGATGTGAAATCCCCGGGCTTAACCTGGGAACTGCATTTGAAACTGGCAGGCTTGAGTCTTGTAGAGGggggTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCcccTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCG
tail seqs.fna
> OY\_9976 D00420 org\_bc=GAGATCAG new\_bc=GAGATCAG bc\_diffs=0
> TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGAGCTCAACTCCAGAATTGCCTTTAAGACTGCATCGCTTGAATCCAGGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGGCTCACTGGACTGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCG
> OY\_9983 D00420 org\_bc=GAGATCAG new\_bc=GAGATCAG bc\_diffs=0
> TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCgcgcgcAGGCGGCTTCTTAAGTCCATCTTAAAAGTGCGGGGCTTAACCCCGTGATGGGATGGAAACTGAGAGGCTGGAGTATCGGAGAGGAAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAACACCGGTGGCGAAGGCGACTTTCTGGACGACAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCGT
> OY\_9987 D00420 org\_bc=GAGATCAG new\_bc=GAGATCAG bc\_diffs=0
> TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTtttAAGTCCGCCGTCAAATCCCAGGGCTCAACTCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCGTAGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAgagaCGAAAGCTAGGGGAGCGAATGGGATTAGATACCCG
> OY\_9989 D00420 org\_bc=GAGATCAG new\_bc=GAGATCAG bc\_diffs=0
> TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCgcgcgcAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTtttGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCG
> OY\_999 D00420 org\_bc=GAGATCAG new\_bc=GAGATCAG bc\_diffs=0
> TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTAGGTGGCTTtttAAGTCCGCCGTCAAATCCCAGGGCTCAACTCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCGTAGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAgagaCGAAAGCTAGGGGAGCGAATGGGATTAGATACCCG
Thanks a lot!
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 8, 2018, 8:16pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/8 "2018-01-08T20:16:53Z")
hm... those certainly look like the correct format but I do notice a bunch of lowercase characters, which _might_ be causing this import issue.
what version of QIIME2 are you using?
Could you try the following?
```auto
cat seqs.fna | tr 'acgt' 'ACGT' > new_seqs.fna
qiime tools import \
–input-path new_seqs.fna \
–output-path seqs.qza \
–type SampleData[Sequences]
```
It is a bit of a shot in the dark — but I can tell you that lowercase nucleotide characters can cause issues with some plugins downstream.
Another possibility is if there are duplicate sequence IDs. That should not occur, but might if you did anything unusual with the qiime1 outputs prior to trying to import (e.g., concatenating multiple seqs.fna files?). I can't really give support on qiime1 commands, but it could help to give some context on the qiime1 commands that you used upstream to generate these files.
Please let me know if that works!
---
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 11, 2018, 5:12pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/9 "2018-01-11T17:12:27Z")
Hello Nicholas,
The above suggestion worked. Thank you very much.
I went back to moving pictures tutorial to try to do taxonomy barplot, so I had to use the feature classifier first, I got a plugin error:
qiime feature-classifier classify-sklearn \
> --i-classifier gg-13-8-99-515-806-nb-classifier.qza
> --i-reads rep-seqs.qza
> --o-classification taxonomy.qza
> Plugin error from feature-classifier
What could be the reason for this?
Thanks a lot
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 11, 2018, 5:34pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/11 "2018-01-11T17:34:29Z")
Hi @Pseudomonas84, can you please copy and paste the full error message output when you run the command with the `--verbose` flag?
---
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 11, 2018, 5:38pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/13 "2018-01-11T17:38:55Z")
Hi Matthew,
Thanks for the reply. I made this input and got the error message below.
qiime feature-classifier classify-sklearn --i-classifier gg-13-8-99-515-806-nb-classifier.qza --i-reads rep-seqs.qza --o-classification taxonomy.qza
Plugin error from feature-classifier:
Debug info has been saved to /tmp/qiime2-q2cli-err-r8vn3hyh.log
Thank you very much!
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 11, 2018, 5:46pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/14 "2018-01-11T17:46:21Z")
Hi @Pseudomonas84, can you please copy and paste the full error message output when you run the command with the `--verbose` flag? For example:
```bash
$ qiime feature-classifier classify-sklearn \
--i-classifier gg-13-8-99-515-806-nb-classifier.qza \
--i-reads rep-seqs.qza \
--o-classification taxonomy.qza \
--verbose
```
Thanks!
---
### Author:  [@Pseudomonas84](https://forum.qiime2.org/u/Pseudomonas84)
#### Post date: [January 11, 2018, 6:13pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/15 "2018-01-11T18:13:25Z")
Hello Matthew,
My VM crashed and I had to restart it and input the command again, and it worked, no errors...weird.
Thanks a lot!
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 11, 2018, 9:29pm UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/16 "2018-01-11T21:29:22Z")
3 off-topic replies have been split into a new topic: [Troubles importing FASTA file](https://forum.qiime2.org/t/troubles-importing-fasta-file/2546)
Please keep replies on-topic in the future.
---
### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 12, 2018, 3:29am UTC](https://forum.qiime2.org/t/seqs-fna-is-not-a-n-qiime1demuxformat-file/2450/17 "2018-02-12T03:29:34Z")
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