Separating two different amplicons from demultiplexed data

I'd recommend exporting and checking out some of your sequences. Are they very short? Are there overhangs or other non-biological sequences present?

You could also try fiddling around with parameters like perc-identity or perc-query-aligned. :violin:

Yes, I believe primers are trimmed out by that command.

Does that help? Let us know if you have more questions!