# Separating Regions - best practices for denoising?

**URL:** https://forum.qiime2.org/t/separating-regions-best-practices-for-denoising/14671
**Category:** User Support
**Tags:** taxonomy, dada2, deblur
**Created:** [April 27, 2020, 7:35pm UTC](https://forum.qiime2.org/t/separating-regions-best-practices-for-denoising/14671 "2020-04-27T19:35:13Z")
**Posts on this page:** 1
**Showing post:** 2

<div class="post-metadata">

### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn)
#### Post date: [April 27, 2020, 7:54pm UTC](https://forum.qiime2.org/t/separating-regions-best-practices-for-denoising/14671/2 "2020-04-27T19:54:27Z")

</div>

Hello Brittany,

Do the separate regions have separate barcodes, so that you can easily split them? If so, I would split them and treat them like two different data sets and never process them together _ever_, just like if you had sequenced 16S and ITS regions.

Dealing with multiple regions is a big challenge, but the good news is that we [great thread going](https://forum.qiime2.org/t/possible-analysis-pipeline-for-ion-torrent-16s-metagenomics-kit-data-in-qiime2/13476/) discussing all parts of the problem. But hopefully you can separate your regions and avoid this messiness.

Colin

---

_[View the full topic](https://forum.qiime2.org/t/separating-regions-best-practices-for-denoising/14671)._