Se-33-manifest is not a(n) SingleEndFastqManifestPhred33 file

[zwbao]

$ qiime tools import \
>   --type 'SampleData[SequencesWithQuality]' \
>   --input-path se-33-manifest \
>   --output-path single-end-demux.qza \
>   --source-format SingleEndFastqManifestPhred33
There was a problem importing se-33-manifest:

  se-33-manifest is not a(n) SingleEndFastqManifestPhred33 file

I am sure that the format of se-33-manifest is right.

$ cat se-33-manifest
sample-id,absolute-filepath,direction
S029,/data/baozw/Shanxitest_18.7.11/data/S029_zy91-A_1_AHNJWYCCXY_S29_L002_R1_001.fastq.gz,forward
S029,/data/baozw/Shanxitest_18.7.11/data/S029_zy91-A_1_AHNJWYCCXY_S29_L002_R2_001.fastq.gz,reverse
S030,/data/baozw/Shanxitest_18.7.11/data/S030_zy91-A_2_AHNJWYCCXY_S30_L002_R1_001.fastq.gz,forward
S030,/data/baozw/Shanxitest_18.7.11/data/S030_zy91-A_2_AHNJWYCCXY_S30_L002_R2_001.fastq.gz,reverse

[thermokarst]

Hey there @zwbao - my guess is that the file has not been saved in UTF-8 encoding — I just copied-and-pasted the CSV snippet you provided and it worked as expected. You could try and run something like:

file --mime se-33-manifest

Which would give you something like this:

se-33-manifest: text/plain; charset=us-ascii

But probably the charset will be something different for you. You will want to configure your editor, spreadsheet tool, or whatever you used to create the manifest as UTF-8 (or ASCII, a subset of UTF-8).

Good luck!

[zwbao]

Thanks a lot ! It works!