Hi, I have three batches of run, I run them in qiime2 seperately.
The I got table-1.qza, table-2.qza, table-3.qza, rep-seqs-1.qza, rep-seqs-2.qza, rep-seqs-3.qza. then I use the code below to merge them.
But after I did these steps, how to draw a bra-plot of them? Because for each run, barcodes are the same. Then final barplot draw needs the sample-metadata. Is there a way out?
Thanks.
qiime feature-table merge \
--i-table1 table-1.qza \
--i-table2 table-2.qza \
--i-table2 table-3.qza \
--o-merged-table table.qza
qiime feature-table merge-seq-data \
--i-data1 rep-seqs-1.qza \
--i-data2 rep-seqs-2.qza \
--i-data1 rep-seqs-3.qza \
--o-merged-data rep-seqs.qza
qiime feature-table tabulate-seqs --i-data rep-seqs.qza --o-visualization rep-seqs.qzv
qiime feature-classifier classify-sklearn --i-classifier gg-13-8-99-515-806-nb-classifier.qza --i-reads rep-seqs.qza --o-classification taxonomy.qza
qiime metadata tabulate --m-input-file taxonomy.qza --o-visualization taxonomy.qzv