# Results consensus when analyzing many V regions

**URL:** https://forum.qiime2.org/t/results-consensus-when-analyzing-many-v-regions/18033
**Category:** User Support
**Created:** [January 12, 2021, 2:54pm UTC](https://forum.qiime2.org/t/results-consensus-when-analyzing-many-v-regions/18033 "2021-01-12T14:54:19Z")
**Posts on this page:** 1
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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [January 12, 2021, 3:19pm UTC](https://forum.qiime2.org/t/results-consensus-when-analyzing-many-v-regions/18033/2 "2021-01-12T15:19:45Z")

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Happy new year to you as well @MiriamGorostidi!

> [@MiriamGorostidi](#):
>
> This Ion Reporter software gives the taxonomic results by regions or using a consensus of all the regions, where the results of each regions are **added up**.

> [@MiriamGorostidi](#):
>
> My questions is if the command `classify-consensus-vsearch` does a similar consensus or not, as I am analyzing 7 regions instead of one.

The "consensus" in this method stands for something else: it means that the taxonomic ID (of each individual read) is assigned based on the consensus taxonomy of the top hits, not a consensus of the separate regions (which sounds more like a sum of the results from each read, rather than a consensus per se based on your description).

But the equivalent could be accomplished, by using the full-length 16S rRNA gene sequences as a reference and analyzing all the data together.

This and other related topics are discussed in this long but informative topic:

> [@Possible Analysis Pipeline for Ion Torrent 16S Metagenomics Kit Data in QIIME2?](https://forum.qiime2.org/t/possible-analysis-pipeline-for-ion-torrent-16s-metagenomics-kit-data-in-qiime2/13476):
>
> Hi QIIME2 community, My colleague @Lauren I have been using the Ion torrent 16S Metagenomics Kit to sequence some clinical samples that we have. Our sample sets are both longitudinal. The kit consists of two primer sets, one for V2-4-8 and one for V3-6,7-9. The products of the reactions are pooled per sample, and then the rest of library prep is completed. Each sample gets its own barcode. Ion Torrent produces single end reads. The issue here is that the reads from each of the 6 variable regio…

Good luck!

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