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Hello Hunter,

Not easily, and perhaps for good reason. This 'two-phase manual blast annotation' will be difficult (or impossible!) for future researchers to replicate, so while it may improve taxonomy for some ASVs, it could also cause a lot of confusion.

(And if I'm worried about this, then you know reviewer three is going to have questions :exploding_head: )

If you want to do it anyway, here's the process:

Export your taxonomy.qza as a .biom file, convert that to a tsv file, edit the ASVs with new taxonomy from blast, then import that back into a Qiime2 taxonomy_edited.qza file.

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