Thank you very much dear @SoilRotifer,
I did not mean any modification onDADA2 artifacts. What I meant is taxonomy and sequences (database) are extracted by Rescript plugin. I need to remove uncultured/ environmental taxa/lineages and sequences.
The filtration methods in Qiime2 tutorial not work this situation because one of requirements is feature table, so in my case I do not need to remove something from my feature table or my rep seq. I just need to remove uncultured ones from database obtained from Rescript.
and
As an example, I need to remove such a taxa and its sequences (with accession numbers) from my database. Please look at the photo below:
- I have no problem with my feature table and repseq at the moment.
Thank you very much indeed in advance.