# RESCRIPT error: Download did not finish. Reason unknown

**URL:** https://forum.qiime2.org/t/rescript-error-download-did-not-finish-reason-unknown/18928
**Category:** Library Support
**Created:** [March 21, 2021, 7:51am UTC](https://forum.qiime2.org/t/rescript-error-download-did-not-finish-reason-unknown/18928 "2021-03-21T07:51:35Z")
**Posts on this page:** 1
**Showing post:** 7

<div class="post-metadata">

### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [March 23, 2021, 2:18pm UTC](https://forum.qiime2.org/t/rescript-error-download-did-not-finish-reason-unknown/18928/7 "2021-03-23T14:18:25Z")

</div>

@avtober, I forgot to mention that you should be aware that the help text also has other helpful information, _e.g._:

> Please be aware of the NCBI Disclaimer and Copyright notice  
> ([Policies and Disclaimers - NCBI](https://www.ncbi.nlm.nih.gov/home/about/policies/)), particularly "run  
> retrieval scripts on weekends or between 9 pm and 5 am Eastern Time  
> weekdays for any series of more than 100 requests". As a rough guide, if  
> you are downloading more than 125,000 sequences, only run this method at  
> those times...

Which could also impact your ability to download data.

One other thought, download your data in chunks as outlined here:

> [@Make 12S reference database using Rescript](https://forum.qiime2.org/t/make-12s-reference-database-using-rescript/18110/4):
>
> Hi @Junli_Zhang, I've got quite a bit of experience in making 12S rRNA gene databases (e.g. for eukaryota, and metazoa). Hopefully, we can help. slight_smile I agree with @Nicholas_Bokulich, there are things beyond our control when it comes to internet connections. Your query does not appear to result in that many sequences, but you can break it up into smaller downloadable chunks within the vertebrates. Here is a query you can use to download Gnathostomata: txid7776[ORGN] AND (12S OR 12S…

by querying separate taxonomic groups. As an example, I ran the following command to download only Rotifera sequences and it worked:

```auto
$ qiime rescript get-ncbi-data \
	--p-query 'txid10190[ORGN] AND (LSU[TITLE] OR 28S[TITLE] or large ribosomal subunit[TITLE] NOT uncultured[TITLE] NOT unidentified[TITLE] NOT unclassified[TITLE])' \
	--o-sequences ncbi-LSU-rotifera-seqs-unfiltered.qza \
	--o-taxonomy ncbi-LSU-rotifera-taxonomy-unfiltered.qza \
	--verbose

Saved FeatureData[Sequence] to: ncbi-LSU-rotifera-seqs-unfiltered.qza
Saved FeatureData[Taxonomy] to: ncbi-LSU-rotifera-taxonomy-unfiltered.qza

```

Since you've listed `28S` as your LSU of interest, I assumed you only wanted to download data from within the Eukaryota, that is `23S` is the LSU for Bacteria / Archaea, which you did not list. Below would be the command for downloading Eukaryote LSU sequences. _Note, this may still be too large of a query, and I'd suggest downloading in chunks as mentioned above._

```auto
qiime rescript get-ncbi-data \
	--p-query 'txid2759[ORGN] AND (LSU[TITLE] OR 28S[TITLE] or large ribosomal subunit[TITLE] NOT uncultured[TITLE] NOT unidentified[TITLE] NOT unclassified[TITLE])' \
	--o-sequences ncbi-LSU-eukaryota-seqs-unfiltered.qza \
	--o-taxonomy ncbi-LSU-eukaryota-taxonomy-unfiltered.qza \
	--verbose

```

You can search the [NCBI Taxonomy page](https://www.ncbi.nlm.nih.gov/taxonomy) to figure out what the `txid` for a given group is.

Finally, you can simply use [RESCRIPt](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494) to download the LSU data from SILVA (an update was recently pushed to the GitHub code to do this for SILVA ver 138.1). Then you can run the following:

```auto
qiime rescript get-silva-data \
	--p-version '138.1' \
	--p-target 'LSURef_NR99' \
	--p-include-species-labels \
	--p-ranks domain domain superkingdom kingdom subkingdom superphylum phylum subphylum infraphylum superclass class subclass infraclass superorder order suborder superfamily family subfamily genus \
	--p-rank-propagation \
	--output-dir silva-138.1-LSU

```

_Note, I listed all available taxonomic ranks to be parsed, as I am not sure which would be most helpful for you in this case. You can remove the ranks that you do not need. Any empty ranks will be filled in by the nearest upper-level rank._

-Cheers!

---

_[View the full topic](https://forum.qiime2.org/t/rescript-error-download-did-not-finish-reason-unknown/18928)._