RESCRIPT error: Download did not finish. Reason unknown

[SoilRotifer (Mike Robeson)]

@avtober

I am looking for eukaryotes really (parasites in snails) but would also like to include fungi and bacteria just incase

In that case, why not use the LSU from SILVA? See here:

Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt

Please consider this tutorial a living document, which may change based upon community feedback and ongoing plugin development. RESCRIPt RESCRIPt (REference Sequence annotation and CuRatIon Pipeline) is a python package and QIIME 2 plugin for formatting, managing, and manipulating sequence reference databases. This package was designed for compiling, manipulating, and evaluating sequence reference databases from SILVA, NCBI, Greengenes, GTDB, and other sources, and for constru…

You can use qiime rescript get-silva-data to download the latest v138.1 LSU database. Just set --p-version 138.1 and --p-target LSURef_NR99 or --p-target LSURef. You can follow along the in the above linked tutorial to perform further filtering and curation of the reference database.