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[colinbrislawn (Colin J Brislawn)]

Hello @Salma_Sarker

You may find this helpful:

Species-level classification

Hi @Soyoung_Yeo, With 16S sequencing it's not always possible to get species-level resolution. The resolution of 16S amplicon sequencing is typically considered reliable at the family or genus level, and species level assignment is sometimes obtainable. This limitation is inherent in the approach - specifically there is not always variation in the short amplicon sequence at the species level (e.g., all members of a genus often have the same 16S sequence for the short fragments that we sequence)…

The Qiime2 devs have done a bunch of work on taxonomy classification! Here is one of their big papers:

Optimizing taxonomic classification of marker-gene amplicon sequences with...