hm… I am not a plant biologist but this additional information makes me think that this solution is unlikely. i.e., I am skeptical that you can use short reads of plastid 16S rRNA genes to get subspecies-level resolution. Yeah, I'm not super confident that this will work, but I wanted to pursue i…

Hi @bpscherer , I am wondering if there is something wrong with your environment? I just ran your commands on my laptop using my qiime2-2020.11 environment: qiime rescript get-ncbi-data \ --p-query '33175[BioProject] OR 33317[BioProject]' \ --p-n-jobs 3 \ --o-sequences ncbi-refseqs-unfiltered…

Ahh, it was indeed that I was using an old version of QIIME. I updated and everything seems good now. Thank you!

Thank you for letting us know @bpscherer . Which version of QIIME was this issue occurring? I think we now require at least version 2020.8 or later. -Cheers! -Mike

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RESCRIPt AttributeError: ‘DNAFASTAFormat’ object has no attribute ‘view

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