# Replace barcode sequence with sample-Id

**URL:** https://forum.qiime2.org/t/replace-barcode-sequence-with-sample-id/3314
**Category:** User Support
**Tags:** demux
**Created:** [March 5, 2018, 2:26pm UTC](https://forum.qiime2.org/t/replace-barcode-sequence-with-sample-id/3314 "2018-03-05T14:26:23Z")
**Posts on this page:** 1
**Showing post:** 9

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### Author: ![M.Amine.Hassan](https://forum.qiime2.org/user_avatar/forum.qiime2.org/m.amine.hassan/32/19996_2.png) [@M.Amine.Hassan](https://forum.qiime2.org/u/M.Amine.Hassan)
#### Post date: [March 8, 2018, 3:42pm UTC](https://forum.qiime2.org/t/replace-barcode-sequence-with-sample-id/3314/9 "2018-03-08T15:42:32Z")

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> [@thermokarst](#):
>
> The manifest format is only for already demultiplexed data. It really sounds like your reads aren’t demultiplexed, but if you could confirm one way or the other, that would be great. If they aren’t demultiplexed, we should be able to import as MultiplexedPairedEndBarcodeInSequence, and follow along with the q2-cutadapt community tutorial to demultiplex these reads.

Indeed, the reads aren't demultiplexed and I could follow the [q2-cutadapt community](https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313) tutorial.  
However, this rises the another [issue](https://forum.qiime2.org/t/cutadapt-with-barcodes-in-reverse-read/3310/3)  
Thanks for the help!

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