No, the actual PCR primers you used for amplification of the V3V4 region. I suspect they might be one of these two primer sets:
| Region | Primer Pair Name | Reference | Fwd primer (5' - 3') | Rev primer (5' - 3') |
|---|---|---|---|---|
| V3V4 | 341F/357wF - 805R | Herlemann et al. 2011 | CCTACGGGNGGCWGCAG | GACTACHVGGGTATCTAATCC |
| V3V4 | 341F/357wF-806R | Lemons et al. 2017 | CCTACGGGNGGCWGCAG | GGACTACHVGGGTWTCTAAT |
This is assuming the sequencing protocol used sequences through the primer, i.e. is present in the 5' region of your reads.
-Mike