# Removing ASV's Found in Extraction Blanks

**URL:** https://forum.qiime2.org/t/removing-asvs-found-in-extraction-blanks/24081
**Category:** User Support
**Created:** [September 4, 2022, 10:46pm UTC](https://forum.qiime2.org/t/removing-asvs-found-in-extraction-blanks/24081 "2022-09-04T22:46:55Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![jwdebelius](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jwdebelius/32/9655_2.png) [@jwdebelius](https://forum.qiime2.org/u/jwdebelius)
#### Post date: [September 5, 2022, 1:59am UTC](https://forum.qiime2.org/t/removing-asvs-found-in-extraction-blanks/24081/2 "2022-09-05T01:59:03Z")

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Hi @Hasti_A,

Welcome to the :qiime2: forums!

Congratulations, you have stumbled into one of thsoe horrifying secrets of microbiome reserach that no one talks about (or everyone talks about).

I'd recommend reading the thread on decontamination:

> [@Discussion: methods for removing contaminants and cross-talk](https://forum.qiime2.org/t/discussion-methods-for-removing-contaminants-and-cross-talk/5626/20):
>
> Hi @natavicula, I’m currently messing around with decontam based on this introduction: [https://benjjneb.github.io/decontam/vignettes/decontam\_intro.html#identifying-contaminants-in-marker-gene-and-metagenomics-data](https://benjjneb.github.io/decontam/vignettes/decontam_intro.html#identifying-contaminants-in-marker-gene-and-metagenomics-data) I also used the prevalence method which detected a few contaminate sequences in my batches, but my question is how did you go forward after identifying the contaminates? The intro just kind of stop after the identification and I can’t seem to find the best way to remove them (unles…

You might also look at a slightly different perscective about well-to-well contamination if you ever used robotic processing in your samples

[https://journals.asm.org/doi/full/10.1128/mSystems.00186-19](https://journals.asm.org/doi/full/10.1128/mSystems.00186-19)

Best,  
Justine

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