Hi @Gil_Sharon! If you are using the EMP Protocol, my understanding is that your demuxed sequences should already have the primers removed (that is part of the protocol). If this isn't the case with your data, and the primers are still present, you can take a look at this forum post to see an option for trimming the sequences during denoising, if using q2-dada2
. We are planning on supporting a general purpose trimming tool at some point in the future (no ETA yet), but this would happen after importing data, not as part of an import step, as you suggested above.
QIIME 2 is interface agnostic, and as a result, provides a few different interfaces for users (see qiime2.org for some nifty screenshots), and those interfaces are all able to define their own specialized functionality. The qiime tools ...
indicates to me that you are using q2cli
, which is our official command line interface. The subcommand tools
is a built-in q2cli
command (one of those "specialized functions" I mentioned above), and isn't a QIIME 2 plugin, which is why you didn't see it in the list of available plugins. Unfortunately we don't yet have detailed documentation online for our interfaces, but, you can learn more about q2cli
but appending --help
to just about any command incantation:
$ qiime tools --help
Usage: qiime tools [OPTIONS] COMMAND [ARGS]...
Tools for working with QIIME 2 files.
Options:
--help Show this message and exit.
Commands:
export Export data from a QIIME 2 Artifact or Visualization.
extract Extract a QIIME 2 Artifact or Visualization archive.
import Import data into a new QIIME 2 Artifact.
peek Take a peek at a QIIME 2 Artifact or Visualization.
view View a QIIME 2 Visualization.
Thanks!