I was trying to train the classifier in QIIME2, but facing difficulty in reference sequences belonging to 97_otus.fasta.
$ qiime tools import --type ‘FeatureData[Sequence]’ --input-path 97_otus.fasta --output-path 97_otus.qza
There was a problem importing 97_otus.fasta:
97_otus.fasta is not a(n) DNAFASTAFormat file:
Invalid characters on line 2 (does not match IUPAC characters for a DNA sequence).
Kindly suggest how I can fix this error.
I have also tried working with other reference sequences i.e., 99_otus.fasta and 85_otus.fasta, which were found to be working fine. But, during one of the following commands shown below (using 99_otus.qza), I got the following error response:
$ qiime feature-classifier extract-reads
Plugin error from feature-classifier:
No matches found
Debug info has been saved to /tmp/qiime2-q2cli-err-l_x18rdb.log
Kindly suggest how I can fix these errors? Thank you very much in advance.