Questions regarding training classifier


(Ruby) #1

Hi guys, I’m totally new to Qiime2. I have a question regarding training the classifier, so after I train the classifier.qza, will the content in my classifier.qza file change? So if I use the same file to do a taxonomy analysis for another sample, will that affect my results? Thank you!

(Nicholas Bokulich) #2

No, that classifier will always work the same way. That is really why the classifier training and classification are split into two steps: you can re-use the trained classifier again and again (as long as the query sequences are appropriate for that classifier, e.g., same amplicon region/primers).

Good luck!

(Ruby) #3

Thank you Nicholas, that’s very helpful!