# Questions about running ANCOM-BC at the ASV level

**URL:** https://forum.qiime2.org/t/questions-about-running-ancom-bc-at-the-asv-level/33972
**Category:** User Support
**Tags:** ancom-bc
**Created:** [February 9, 2026, 9:05pm UTC](https://forum.qiime2.org/t/questions-about-running-ancom-bc-at-the-asv-level/33972 "2026-02-09T21:05:06Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![rylee650](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/r/bc79bd/32.png) [@rylee650](https://forum.qiime2.org/u/rylee650)
#### Post date: [February 9, 2026, 9:05pm UTC](https://forum.qiime2.org/t/questions-about-running-ancom-bc-at-the-asv-level/33972/1 "2026-02-09T21:05:07Z")

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# 

After referring to a few forum posts on ANCOM-BC, I decided to run ANCOM-BC at the ASV level in addition to using my table collapsed at the L7 level for my fungal ITS data. After assigning taxonomy to the individual feature IDs in R I noticed some of my treatment groups have the exact same taxa enriched with different log fold change values.

The issue I am having is how to properly visualize this data for a presentation. I had made a heat map in R to show the magnitude change of each taxa, but I am not sure how to go about this for the ASV level. Should I just keep all ASVs in individual rows, or if there some way to combine the taxa to get one LFC value?

I appreciate any insight as I am still relatively new at this!

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### Author: ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)
#### Post date: [February 9, 2026, 9:20pm UTC](https://forum.qiime2.org/t/questions-about-running-ancom-bc-at-the-asv-level/33972/2 "2026-02-09T21:20:49Z")

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Hello!

Usually, I check if my results with collapsed data agree with the test at the ASV level. If yes, I just use output from collapsed test. If ASVs behave differently from taxa (ASVs from the same taxa have the opposite signs of LFC values, or just too different), then I use first 5-6 characters from ASV Id and taxonomy (Lactobacillus\_abc123, Lactobacillus\_def456).

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [March 13, 2026, 3:21am UTC](https://forum.qiime2.org/t/questions-about-running-ancom-bc-at-the-asv-level/33972/3 "2026-03-13T03:21:45Z")

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