Hello, I have some questions about starting with PE sequencing data. I have sequencing data. They are 16S MiSeq using EMP primers and barcodes. I follow the instructions from this tutorials ( “Atacama soil microbiome” tutorial — QIIME 2 2019.10.0 documentation ) I have some questions: 1> The first s…

Hi @moonlight , There's a lot of different questions going on here so let's break them down, in the future you may consider separating some of these questions in separate threads, not only is that easier for archiving and searching but from experience you will get faster responds from the community…

Hello Mehrod, Thanks for you reply. Just a follow up of your reply. I did some test 1>In my dataset, If I don't add the parameters "--p-rev-comp-barcodes " and "--p-rev-comp-mapping-barcodes". The script give me errors 2>If I only have parameter "--p-rev-comp-barcodes", the script gives me error. …

[image] moonlight: If you do twice reverse compliment, this means you don’t need to do this. :wink: False (according to the method defaults). The mapping barcodes must be reverse-complemented to be considered valid golay barcodes. Then the barcodes must be reverse-complemented to match your m…

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