qiime2R: object ‘tax’ not found

[kristiny (Kristin)]

Hi,

I’m having issues getting my data into R using qza_to_phyloseq. I am getting the error message:

Error in qza_to_phyloseq(“settling_itags_table_filtered_rarefied_16921_json.qza”, :
object ‘tax’ not found

I produced the original table in qiime2, then converted the file to .txt, manipulated some things (filtered and rarefied the table using qiime1 scripts, but nothing to the format otherwise), then converted the file back to a .qza file. Is something wrong with the format of the .qza file?

[ebolyen (Evan Bolyen)]

I produced the original table in qiime2, then converted the file to .txt, manipulated some things (filtered and rarefied the table using qiime1 scripts, but nothing to the format otherwise), then converted the file back to a .qza file. Is something wrong with the format of the .qza file?

Could you provide the commands you used to create that?

Also, what does qiime tools validate say about your .qza file?