QIIME2 Use DADA2

HI,
I am using QIIME2 for 16S faecal sample meta analysis,
I am at DADA2 step but after I wrote these following command
qiime dada2 denoise-paired
–i-demultiplexed-seqs demux-paired-end.qza
–o-table table
–o-representative-sequences rep-seqs
–o-denoising-stats dada2stats
–p-trim-left-f 10
–p-trim-left-r 10
–p-trunc-len-f 240
–p-trunc-len-r 240
I read this message : There was a problem with the command:
(1/1) Invalid value for ‘–i-demultiplexed-seqs’: ‘demux-paired-end.qza’ is
not a valid filepath

Can you help me to continue the analysis?

Hi!

Looks like dada2 plugin can not find the input artifact (‘demux-paired-end.qza’) you provided. Please, double check that you are providing the right path to your demultiplexed sequences. If you will not be able to resolve the issue, provide a screenshot of the directory in which you are working to help to troubleshoot the problem.

2 Likes


Now the situation is that…I don’t know why does it not go on?

Hello!
You have several typos in your command - you should add .qza to outputs (table, rep-seqs and dada2stats).
Also, could you run ls and provide a screenshot? That will list all files in your working directory in terminal