I am using QIIME2 for 16S faecal sample meta analysis,
I am at DADA2 step but after I wrote these following command
qiime dada2 denoise-paired
–i-demultiplexed-seqs demux-paired-end.qza
–o-table table
–o-representative-sequences rep-seqs
–o-denoising-stats dada2stats
–p-trim-left-f 10
–p-trim-left-r 10
–p-trunc-len-f 240
–p-trunc-len-r 240
I read this message : There was a problem with the command:
(1/1) Invalid value for ‘–i-demultiplexed-seqs’: ‘demux-paired-end.qza’ is
not a valid filepath

Can you help me to continue the analysis?


Looks like dada2 plugin can not find the input artifact (‘demux-paired-end.qza’) you provided. Please, double check that you are providing the right path to your demultiplexed sequences. If you will not be able to resolve the issue, provide a screenshot of the directory in which you are working to help to troubleshoot the problem.


Now the situation is that…I don’t know why does it not go on?

You have several typos in your command - you should add .qza to outputs (table, rep-seqs and dada2stats).
Also, could you run ls and provide a screenshot? That will list all files in your working directory in terminal