Hi @Karthika,
Thanks for your patience here! Happy to address your questions below.
One cautionary point I'd like to bring up about this is that you'll only be able to utilize dada2 and/or deblur for denoising on your 16S samples (but not your WGS samples). Depending on what your analysis requires, this may or may not be relevant - but it's worth bringing up before moving forward.
You'll need to import, denoise, and demultiplex your single end and paired end reads separately - which will allow you to move forward in your analysis. Check out this forum post that addresses this same question in further detail (separating single end and paired end reads and any relevant comparisons between the two datasets).
Hope this helps!
Cheers