# Qiime2-Picrust2 outputs

**URL:** https://forum.qiime2.org/t/qiime2-picrust2-outputs/26151
**Category:** PICRUSt2
**Tags:** picrust
**Created:** [April 18, 2023, 2:57pm UTC](https://forum.qiime2.org/t/qiime2-picrust2-outputs/26151 "2023-04-18T14:57:14Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![hharder](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/h/e5b9ba/32.png) [@hharder](https://forum.qiime2.org/u/hharder)
#### Post date: [April 18, 2023, 2:57pm UTC](https://forum.qiime2.org/t/qiime2-picrust2-outputs/26151/1 "2023-04-18T14:57:14Z")

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I have used the q2-picrust2 plugin using the following code:

> qiime picrust2 full-pipeline --i-table sequence\_variants.qza --i-seq rep-seqs.qza --output-dir q2-picrust2-output --p-threads 1 --p-hsp-method mp -p-max-nsti 2 --verbose

It worked perfectly, and I got an output folder containing three qza files: ec\_metagenome.qza, ko\_metagenome.qza, and pathway\_abundance.qza. I now want to do downstream analysis using the ggpicrust2 R package. However, the instructions ([https://cran.r-project.org/web/packages/ggpicrust2/readme/README.html](https://cran.r-project.org/web/packages/ggpicrust2/readme/README.html)) specify to use a file called "picrust2\_out/KO\_metagenome\_out/pred\_metagenome\_unstrat.tsv/pred\_metagenome\_unstrat.tsv" from the output. Where are these files located and how can I access them? I only have qza files. Do I need to run the analysis in individual steps vs. using full-pipeline? Do I need to use the standalone Picrust2 tool vs. the Qiime plugin? I tried to export the qza file to see the files in it but I couldn't find anything resembling pred\_metagenome\_unstrat.

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### Author: ![cherman2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cherman2/32/17129_2.png) [@cherman2](https://forum.qiime2.org/u/cherman2)
#### Post date: [April 18, 2023, 5:11pm UTC](https://forum.qiime2.org/t/qiime2-picrust2-outputs/26151/3 "2023-04-18T17:11:08Z")

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Hi @hharder,  
I believe you will want to unzip your ko\_metagenome.qza and go into the data folder. There should be a feature-table.biom. You can then convert that biom file to a tsv using the` biom convert command` .

Hope that helps!  
🐢
