Qiime2 data importing problem

[ion0327 (hokyoung)]

Hello everyone

I am a beginner in Qiime2, and the whole microbiome analysis. I am now having trouble importing data.

The data I got from the lab is from 34 patients.
We got 2 different tissue samples from each patient.

As far as I know, they used illumina sequencing machine and did a paired-end sequencing.

The data file looks like the one below.

The first 4 samples seems to be from a patient, and from two different part of the body (A and B)

I have tried importing the data as Casava 1.8 paired-end demultiplexed fastq, but it said that they could not recognize 4 samples (from two parts of the body).

Do I need to assemble all 4 data files to a single file?

Or what other method can I use to import all the samples?

Thank you very much

[lizgehret]

Hi @ion0327,

Welcome to the forum!

Before moving forward with your question, can you please provide the following details?

• Version of QIIME 2 you are running, and how it is installed (e.g. Virtualbox, conda, etc.)
• What is the exact command (or commands) that you ran? Copy and paste please.
• What is the exact error message you received, if applicable? If you didn't run the command with the --verbose flag, please re-run and copy-and-paste the results.

Thanks!