# Qiime2 2018.4 and 2018.11 greengene

**URL:** https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250
**Category:** User Support
**Tags:** greengenes
**Created:** [December 3, 2018, 3:36am UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250 "2018-12-03T03:36:20Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![Clara](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/c/d26b3c/32.png) [@Clara](https://forum.qiime2.org/u/Clara)
#### Post date: [December 3, 2018, 3:36am UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250/1 "2018-12-03T03:36:20Z")

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Hi there,

I have a question here, previously when I analysed my sequencing data, in the step where I generated my otu table, I used greengene in the version 2018.4. However, when I re-run my qiime2, the same step has been updated to 2018.11. My otu table has therefore change with REDUCE number of genera. May I know in this instant, should I use the previous version of otu table or the latest one?

Thanks.

Clara

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [December 3, 2018, 4:57pm UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250/2 "2018-12-03T16:57:52Z")

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Hi there @Clara! Greenegenes hasn't been updated in many years --- I suspect that you might have some subtle differences in the commands run between the two versions. How about you [compare provenance](https://forum.qiime2.org/t/how-to-read-provenance-in-an-artifact-file/7257/2) for the two tables to see what has changed? If you need help can you run `feature-table summarize` on the two tables and share here? Thanks!

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### Author: ![Clara](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/c/d26b3c/32.png) [@Clara](https://forum.qiime2.org/u/Clara)
#### Post date: [December 3, 2018, 7:39pm UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250/3 "2018-12-03T19:39:21Z")

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Hi @thermokarst, here is the link that I found "different" when running the command line, that is why I asked about greengene update

wget -O "gg-13-8-99-515-806-nb-classifier.qza" [https://data.qiime2.org/\*\*2018.4\*\*/common/gg-13-8-99-515-806-nb-classifier.qza](https://data.qiime2.org/ **2018.4** /common/gg-13-8-99-515-806-nb-classifier.qza)

wget   
-O "gg-13-8-99-515-806-nb-classifier.qza"   
[https://data.qiime2.org/\*\*2018.11\*\*/common/gg-13-8-99-515-806-nb-classifier.qza](https://data.qiime2.org/ **2018.11** /common/gg-13-8-99-515-806-nb-classifier.qza)

Is it not because of this?

And because the two ran were using the same table-dada2, so I would think that the otu table generated will be the same?

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [December 4, 2018, 2:27pm UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250/4 "2018-12-04T14:27:02Z")

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Hey @Clara! Ah yeah, those are links to pre-trained classifiers, not the original greengenes database itself. It looks like some of the default classifier parameters [changed in 2018.6](https://forum.qiime2.org/t/qiime-2-2018-6-release-is-now-live/4692) - is it possible that that is what you are seeing? You could confirm by retraining a greengenes-based classifier using the old settings.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [January 4, 2019, 8:39pm UTC](https://forum.qiime2.org/t/qiime2-2018-4-and-2018-11-greengene/7250/5 "2019-01-04T20:39:10Z")

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