Qiime tools-import help!

[FabiolaE1 (Fabiola Estrada)]

Hello!

I am trying to import my data into QIIME2.2022.8 and I keep getting this error & not sure what to do. If anyone can help I will really appreciate it.

(qiime2-2022.8) RR179798IP01:~ eys900$ qiime tools import \

--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path /Volumes/LaCie/FE.16S/DataFE.txt
--output-path /Volumes/LaCie/Sequences.qza
--input-format PairedEndFastqManifestPhred33V2

There was a problem importing /Volumes/LaCie/FE.16S/DataFE.txt:

/Volumes/LaCie/FE.16S/DataFE.txt is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 2 and column "forward-absolute-filepath" (sample "FAB2") has already been registered on line 1 and column "forward-absolute-filepath" (sample "FAB1").

Thank you!

[cherman2 (Chloe Herman)]

Hi @FabiolaE1,
Looks like this is an issue with your manifest file. More specifically, you have the same forward-absolute-filepath for 2 samples. The error says that it is your first and second samples. With manifests each sample needs to have its own unique forward and reserves file(at least for paired end reads).

Let me know if you need more help diagnosing, if so send your manifest file so I can take a look.