# qiime quality-control exclude-seqs message
**URL:** https://forum.qiime2.org/t/qiime-quality-control-exclude-seqs-message/21295
**Category:** Technical Support
**Created:** [November 10, 2021, 9:26am UTC](https://forum.qiime2.org/t/qiime-quality-control-exclude-seqs-message/21295 "2021-11-10T09:26:49Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@ezgi](https://forum.qiime2.org/u/ezgi)
#### Post date: [November 10, 2021, 9:26am UTC](https://forum.qiime2.org/t/qiime-quality-control-exclude-seqs-message/21295/1 "2021-11-10T09:26:49Z")
Hi,
I am running the following commands to remove the host seqs from rep-seqs:
```auto
qiime quality-control exclude-seqs \
--i-query-sequences rep-seqs.qza \
--i-reference-sequences hg38.sequences.qza \
--p-method blast \
--p-threads 12 \
--p-perc-identity 0.97 \
--p-perc-query-aligned 0.97 \
--o-sequence-hits hits.qza \
--o-sequence-misses misses.qza\
--verbose
qiime feature-table tabulate-seqs \
--i-data hits.qza \
--o-visualization hits.qzv \
--verbose
qiime feature-table tabulate-seqs \
--i-data misses.qza \
--o-visualization misses.qzv
```
`misses.qzv/.qza` is produced, but not `hits.qzv/.qza` and `misses.qza` includes the same number of seqs with `rep-seqs.qza`.
QIIME2 produces this message:
```auto
Warning: [blastn] Examining 5 or more matches is recommended
/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/skbio/io/registry.py:548: FormatIdentificationWarning: <_io.BufferedReader name='/t
mp/qiime2-archive-q0ul0x0v/ad0f9080-2fde-4162-b59a-f06be14c7794/data/dna-sequences.fasta'> does not look like a fasta file
% (file, fmt), FormatIdentificationWarning)
Traceback (most recent call last):
File "/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__
results = action(**arguments)
File "", line 2, in tabulate_seqs
File "/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
output_types, provenance)
File "/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/action.py", line 452, in _callable_executor_
ret_val = self._callable(output_dir=temp_dir, **view_args)
File "/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2_feature_table/_summarize/_visualizer.py", line 46, in tabulate_seqs
seq_len_stats = _compute_descriptive_stats(seq_lengths)
File "/home/miniconda3/envs/qiime2-2020.8/lib/python3.6/site-packages/q2_feature_table/_summarize/_visualizer.py", line 206, in _compute_descript
ive_stats
raise ValueError('No values provided.')
ValueError: No values provided.
Plugin error from feature-table:
No values provided.
```
What could be the problem here?
Thank you very much for your help!
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [November 15, 2021, 2:30pm UTC](https://forum.qiime2.org/t/qiime-quality-control-exclude-seqs-message/21295/2 "2021-11-15T14:30:37Z")
The error message you posted above is a little confusing, it looks to me like its multiple messages concatenated together, can you confirm that? The first line (or two?) are from the `exclude-seqs` output, while the rest appear to be from the first call to `tabulate-seqs` - the error is telling you that the `hits.qza` file, while present, is completely empty.
---
### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [December 16, 2021, 8:31pm UTC](https://forum.qiime2.org/t/qiime-quality-control-exclude-seqs-message/21295/3 "2021-12-16T20:31:29Z")
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