qiime longitudinal volatility

User Support
1

Hi,

I am trying to run the qiime longitudinal volatility command with bray_curtis_distance_matrix.qza:

qiime longitudinal volatility --m-metadata-file mapping_file_RS_volatility_analysis_july12_2021.txt --m-metadata-file bray_curtis_distance_matrix.qza --p-default-metric bray_curtis --p-default-group-column Diet --p-state-column Time --p-individual-id-column SubjectID --o-visualization volatility_diet_bray_curtis.qzv

But I am getting:
There was an issue with viewing the artifact bray_curtis_distance_matrix.qza as QIIME 2 Metadata:

Artifact <artifact: DistanceMatrix uuid: 58dff42e-20a9-4e45-aa3f-8893b41e5e0e> cannot be viewed as QIIME 2 Metadata.

Am I using the correct file/format for beta diversity?

Thanks!

2

Hello @idapantoja,

Double check the documentation for this plugin!
https://docs.qiime2.org/2021.4/plugins/available/longitudinal/volatility/

Looks like this command needs a --i-table, and the --m-metadata-file can't be a distance matrix.

6

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