Hello! I have a question regarding the use of
qiime feature-table filter-features
I have 4 replicates for each experimental condition from which I have extracted DNA (in triplicate) and performed 16s sequencing. So, I have a total of 12 data points her experimental condition (4 experimental replicates X 3 extraction replicates).
I noticed that there are a lot of singletons in my data and would like to filter the rarefied table. I have gone through the tutorial and it is really great in helping me filter out the singletons, but across all the samples at the same time. I was wondering if it would be possible to do the same within the 12 replicates of each experimental condition (within each group/description column, so to speak). I would really appreciate your help with the same. Thank you!