# qiime dada2 denoise-paired never ending = stay at remove chimera without errors **URL:** https://forum.qiime2.org/t/qiime-dada2-denoise-paired-never-ending-stay-at-remove-chimera-without-errors/24321 **Category:** Technical Support **Tags:** dada2 **Created:** [September 30, 2022, 7:38am UTC](https://forum.qiime2.org/t/qiime-dada2-denoise-paired-never-ending-stay-at-remove-chimera-without-errors/24321 "2022-09-30T07:38:08Z") **Posts on this page:** 1 **Showing post:** 3
### Author: ![lizgehret](https://forum.qiime2.org/user_avatar/forum.qiime2.org/lizgehret/32/17128_2.png) [@lizgehret](https://forum.qiime2.org/u/lizgehret) #### Post date: [October 4, 2022, 5:59pm UTC](https://forum.qiime2.org/t/qiime-dada2-denoise-paired-never-ending-stay-at-remove-chimera-without-errors/24321/3 "2022-10-04T17:59:12Z")
Hi @marioncdl, Thanks for reaching out and providing those details! ```auto Self-consistency loop terminated before convergence. ``` It looks like this is where the issue is coming from. Here are a few forum posts that discuss this issue and contain a couple of solutions that you can try: > [@Self-consistency loop terminated before convergence.An error was encountered while running DADA2 in R (return code 1)](https://forum.qiime2.org/t/self-consistency-loop-terminated-before-convergence-an-error-was-encountered-while-running-dada2-in-r-return-code-1/3532): > > Hello, I have been getting the following error after the denoising step: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. I use this command: qiime dada2 denoise-paired --i-demultiplexed-seqs paired-end-demux.qza --o-table table --o-representative-sequences rep-seqs --p-trim-left-f 5 --p-trim-left-r 5 --p-trunc-len-f 205 --p-trunc-len-r 205 I check the error log: cat /var/folders/py/xrlp2jks4jq1kqx3mwf99tk… > [@Dada2 Error Return Code 1, Paired-End Seqs](https://forum.qiime2.org/t/dada2-error-return-code-1-paired-end-seqs/2680): > > Hi, I have been running dada2 on paired-end sequences, and had it prematurely terminate. I ran it again with the verbose command and here is the output: R version 3.4.1 (2017-06-30) Loading required package: Rcpp DADA2 R package version: 1.6.0 Filtering … Learning Error Rates 2a) Forward Reads Initializing error rates to maximum possible estimate. Sample 1 - 111645 reads in 64913 unique sequences. Sample 2 - 104707 reads in 58972 unique sequences. Sample 3 - 117878 reads in 68120 u… > [@Dada2: Self-consistency loop terminated before convergence](https://forum.qiime2.org/t/dada2-self-consistency-loop-terminated-before-convergence/1716): > > Hello Everyone, I am running a rather large 16S data set using dada2 and the following command: qiime dada2 denoise-paired --i-demultiplexed-seqs 16S\_demux-paired-end.qza --o-table 16S\_dada2\_table --o-representative-sequences 16S\_rep-seqs --p-trunc-len-f 250 --p-trunc-len-r 238 --verbose --p-n-threads 0 --p-n-reads-learn 2000000 I received this warning: Warning message: In dada(drpsF, err = NULL, selfConsist = TRUE, multithread = multithread) : Self-consistency loop terminated before co… Give the suggestions in these posts a try, and let us know if you're still running into any issues! Cheers 🦎 --- _[View the full topic](https://forum.qiime2.org/t/qiime-dada2-denoise-paired-never-ending-stay-at-remove-chimera-without-errors/24321)._