Hello,
I used my denosiing script on my 16S data and it failed. The error that I am getting is
denoise paired end for Afaf
Usage: qiime dada2 denoise-paired [OPTIONS]
Try "qiime dada2 denoise-paired --help" for help.
Error: no such option: --p-max-ee-f
Here is the code I used
qiime dada2 denoise-paired --i-demultiplexed-seqs 16S_demux.qza/per_sample_sequences.qza --p-n-threads 28 --p-max-ee-f 2.0 --p-max-ee-r 2.0 --p-trim-left-f 0 --p-trim-left-r 0 --p-trunc-len-f 0 --p-trunc-len-r 0 --o-representative-sequences 16S_rep-seqs-dada2_f1.qza --o-table 16S_table-dada2_f1.qza --o-denoising-stats 16S_stats-dada2_f1.qza --verbose
I already checked and the command --p-max-ee-f 0 is available in qiime2-2022.8 (which is the version I am using)
Please guide me through this, how can the command be available in the qiime2 version and yet I am getting an error that says it is not available