# Qiime cutadapt demux-paired

**URL:** https://forum.qiime2.org/t/qiime-cutadapt-demux-paired/6004
**Category:** User Support
**Created:** [September 12, 2018, 5:02pm UTC](https://forum.qiime2.org/t/qiime-cutadapt-demux-paired/6004 "2018-09-12T17:02:07Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![laoshiren](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/l/df788c/32.png) [@laoshiren](https://forum.qiime2.org/u/laoshiren)
#### Post date: [September 12, 2018, 5:02pm UTC](https://forum.qiime2.org/t/qiime-cutadapt-demux-paired/6004/1 "2018-09-12T17:02:07Z")

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I'm running 'qiime cutadapt demux-paired', but I got some error...  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/commands.py", line 274, in **call**  
results = action(\*\*arguments)  
File "", line 2, in demux\_paired  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/qiime2/sdk/action.py", line 231, in bound\_callable  
output\_types, provenance)  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/qiime2/sdk/action.py", line 362, in _callable\_executor_  
output\_views = self.\_callable(\*\*view\_args)  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/q2\_cutadapt/\_demux.py", line 136, in demux\_paired  
return \_demux(seqs, forward\_barcodes, error\_rate, untrimmed)  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/q2\_cutadapt/\_demux.py", line 109, in \_demux  
run\_command(cmd)  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/site-packages/q2\_cutadapt/\_demux.py", line 33, in run\_command  
subprocess.run(cmd, check=True)  
File "/home/qiime2/miniconda/envs/qiime2-2018.8/lib/python3.5/subprocess.py", line 398, in run  
output=stdout, stderr=stderr)  
subprocess.CalledProcessError: Command '['cutadapt', '--front', 'file:/tmp/tmp0dwk8czi', '--error-rate', '0.1', '-o', '/tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-rkizuefd/{name}.1.fastq.gz', '--untrimmed-output', '/tmp/q2-MultiplexedPairedEndBarcodeInSequenceDirFmt-la8mbalc/forward.fastq.gz', '-p', '/tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-rkizuefd/{name}.2.fastq.gz', '--untrimmed-paired-output', '/tmp/q2-MultiplexedPairedEndBarcodeInSequenceDirFmt-la8mbalc/reverse.fastq.gz', '/tmp/qiime2-archive-mw6bbrxu/20956bdb-6f4e-4982-b5e7-f2f7b909d336/data/forward.fastq.gz', '/tmp/qiime2-archive-mw6bbrxu/20956bdb-6f4e-4982-b5e7-f2f7b909d336/data/reverse.fastq.gz']' returned non-zero exit status 1

Plugin error from cutadapt:

Command '['cutadapt', '--front', 'file:/tmp/tmp0dwk8czi', '--error-rate', '0.1', '-o', '/tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-rkizuefd/{name}.1.fastq.gz', '--untrimmed-output', '/tmp/q2-MultiplexedPairedEndBarcodeInSequenceDirFmt-la8mbalc/forward.fastq.gz', '-p', '/tmp/q2-CasavaOneEightSingleLanePerSampleDirFmt-rkizuefd/{name}.2.fastq.gz', '--untrimmed-paired-output', '/tmp/q2-MultiplexedPairedEndBarcodeInSequenceDirFmt-la8mbalc/reverse.fastq.gz', '/tmp/qiime2-archive-mw6bbrxu/20956bdb-6f4e-4982-b5e7-f2f7b909d336/data/forward.fastq.gz', '/tmp/qiime2-archive-mw6bbrxu/20956bdb-6f4e-4982-b5e7-f2f7b909d336/data/reverse.fastq.gz']' returned non-zero exit status 1

See above for debug info.

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<div class="post-metadata">

### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [September 12, 2018, 11:39pm UTC](https://forum.qiime2.org/t/qiime-cutadapt-demux-paired/6004/2 "2018-09-12T23:39:00Z")

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This has been merged into an existing topic: [“EMP protocol” multiplexed paired-end fastq file](https://forum.qiime2.org/t/emp-protocol-multiplexed-paired-end-fastq-file/5981)

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### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [September 12, 2018, 11:39pm UTC](https://forum.qiime2.org/t/qiime-cutadapt-demux-paired/6004/3 "2018-09-12T23:39:00Z")

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