# QIIME 2 2026.4 environment files

**URL:** <https://forum.qiime2.org/t/qiime-2-2026-4-environment-files/34077>\
**Category:** Announcements\
**Tags:** environment-files\
**Created:** [March 25, 2026, 11:00am UTC](https://forum.qiime2.org/t/qiime-2-2026-4-environment-files/34077 "2026-03-25T11:00:56Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![q2d2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/q2d2/32/613_2.png) [@q2d2](https://forum.qiime2.org/u/q2d2)\
**Post date:** [March 25, 2026, 11:00am UTC](https://forum.qiime2.org/t/qiime-2-2026-4-environment-files/34077/1 "2026-03-25T11:00:56Z")

</div>

## Hello QIIME 2 Developer Community! 👋🏼 :qiime2:

Below is the list of guaranteed interface changes, plugin API changes, and environment file updates for the 2026.4 QIIME 2 release. You can use the linked environment files for each distribution to ensure compatibility with any plugin you're developing against the relevant distribution it should be tested/used within. Note that these 'seed' environment files won't change between now and the 2026.4 release, but the full environment files that conda will resolve at any given installation will change. This shouldn't impact your development process, as all of the core dependencies in these 'seed' files will remain the same.

* * *

**Interface Changes 📺**

* * *

**Plugin API Changes 🔌**

* * *

**Environment File Changes 🗃**

**TINY**

[linux environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/tiny/passed/seed-environment-conda-linux.yml)  
[macos environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/tiny/passed/seed-environment-conda-osx.yml)

- biom-format=2.1.17
- h5py=3.15.1
- hdf5=1.14.6
- networkx=3.6.1
- numpy=2.4.2
- pandas=2.3.3
- parsl=2026.2.23
- python=3.12
- r-base=4.5.2
- r-reticulate=1.45.0
- scikit-bio=0.7.2
- scikit-learn=1.7.1
- scipy=1.17.1
- tzlocal=5.3.1
- unifrac-binaries=1.5.1
- unifrac=1.5.1

**AMPLICON** (_plus all changes from tiny_)

[linux environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/amplicon/passed/seed-environment-conda-linux.yml)  
[macos environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/amplicon/passed/seed-environment-conda-osx.yml)

- blast=2.17.0
- fastp=1.0.1
- matplotlib=3.10.8
- ncbi-datasets-pyclient=18.18.0
- patsy=1.0.2
- pillow=12.1.1
- r-vegan=2.7\_3
- rpy2=3.6.4
- samtools=1.22.1
- seaborn=0.13.2
- sepp=4.5.6
- statsmodels=0.14.6
- tqdm=4.67.3

**MOSHPIT** (_plus all changes from tiny_\*)

[linux environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/moshpit/passed/seed-environment-conda-linux.yml)  
[macos environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/moshpit/passed/seed-environment-conda-osx.yml)

\*_ **NOTE** _: _The required r-base version for these updates led to a downgrade from 4.5.2 (used in tiny and amplicon) to 4.4.2. The only overlapping plugin affected in both amplicon and moshpit is q2-quality-control (via decontam). The decontam versions from both r-base releases were reviewed and showed no breaking changes, bugs, or meaningful behavioral differences, so usage of q2-quality-control should remain consistent between amplicon and moshpit._

- bigscape=2.0.2
- biopython=1.86
- bowtie2=2.5.5
- das\_tool=1.1.7
- eggnog-mapper=2.1.12
- entrezpy=2.1.3
- matplotlib=3.9.1
- metabat2=2.18
- multiqc=1.33
- ncbi-datasets-pyclient=18.18.0
- pyarrow=16.1.0
- pysam=0.23.3
- r-base=4.4.2
- samtools=1.22.1
- seaborn=0.13.2
- semibin=2.2.0
- sepp=4.5.6
- spades=4.2.0
- tqdm=4.67.3
- vamb=5.0.4

**PATHOGENOME** (_plus all changes from tiny_)

[linux environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/pathogenome/passed/seed-environment-conda-linux.yml)  
[macos environment file](https://raw.githubusercontent.com/qiime2/distributions/refs/heads/dev/2026.4/pathogenome/passed/seed-environment-conda-osx.yml)

- genomad=1.11.2
- matplotlib=3.10.8
- prodigal=2.6.3
- pyarrow=16.1.0
- seaborn=0.13.2
- screed=1.0.4
- spades=4.2.0
- tqdm=4.67.3