# q2-woltka metadata and sample ID

**URL:** https://forum.qiime2.org/t/q2-woltka-metadata-and-sample-id/23404
**Category:** Library Support
**Tags:** metadata
**Created:** [June 20, 2022, 6:38pm UTC](https://forum.qiime2.org/t/q2-woltka-metadata-and-sample-id/23404 "2022-06-20T18:38:45Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![Julian\_Rozenberg](https://forum.qiime2.org/user_avatar/forum.qiime2.org/julian_rozenberg/32/13150_2.png) [@Julian\_Rozenberg](https://forum.qiime2.org/u/Julian_Rozenberg)
#### Post date: [June 20, 2022, 6:38pm UTC](https://forum.qiime2.org/t/q2-woltka-metadata-and-sample-id/23404/1 "2022-06-20T18:38:45Z")

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Dear fellowa  
I am trying to understand how to add a valid metadata to .sam files.  
Apparently, it is required that  
[https://library.qiime2.org/plugins/q2-woltka/42/](https://library.qiime2.org/plugins/q2-woltka/42/)  
" \* The input alignment must be **multiplexed** , with sample ID and an underscore (`_`) prefixing every read ID."  
However, It is clear that I can concatenate fasta, bit is not clear how to add sampleID\_ to read ID sam files.  
I was rtying to add --rg-id ID\_ to bowtie to and it does not work  
Even I can read .sam files and do .table.qza with woltka, this is useless without metadata files that i can use with them.

Thank you in advance for you help.  
Julian Rozenberg, PhD.

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