Missing option '--i-kmer-map'. This document seems to have a mistake on Welcome to Sidle — q2-sidle 2020.8 documentation when l do “ Reconstructing the Phylogenetic Tree” There is no one option --i-regional-alignment but '--i-kmer-map which one is right？ I would like to ask the length of the km…

Hi @lam , Welcome to the :qiime2: forum! [image] lam: Missing option '--i-kmer-map'. This document seems to have a mistake on Welcome to Sidle — q2-sidle 2020.8 documentation when l do “ Reconstructing the Phylogenetic Tree” There is no one option --i-regional-alignment but '--i-kmer-map whi…

Thank you so much for your reply. my primers find in this article Combining 16S rRNA gene variable regions enables high-resolution microbial community profiling - PMC Table 1 Region # amplified Forward primer Reverse primer Sequence Position Sequence Position 1 196415 5′-TGGCGGACGGGTGAGTAA-3′ 7…

Hi @lam , When I ran those, I think these are the parameters i used, based on the exaample from the SMURF repo 74f_315r: fwd: TGGCGGACGGGTGAGTAA rev: CTGCTGCCTCCCGTAGGA length: 215 316f_484r: fwd: TCCTACGGGAGGCAGCAG rev: TATTACCGCGGCTGCTGG length: 125 486f_650r: fwd: CAGCAGCCGCGGTAATA…

thank you for your work very much！ That's what I was thinking but there is a problem，only use one region to seems like will discard some asvs

Hi @lam , You will, but the algorithms also aren't designed to handle multiple regions. I might also personally consider whether you need PICRUSt at this point - I tend to find mixed reactions in the broader community and I might see what your data looks like before diving in there. Best, Justine …

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