# q2-sapienns: total sum scaling

**URL:** https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138
**Category:** Library Support
**Created:** [July 20, 2023, 2:55pm UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138 "2023-07-20T14:55:59Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![em\_joyce](https://forum.qiime2.org/user_avatar/forum.qiime2.org/em_joyce/32/16290_2.png) [@em\_joyce](https://forum.qiime2.org/u/em_joyce)
#### Post date: [July 20, 2023, 2:55pm UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138/1 "2023-07-20T14:55:59Z")

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Hello!

I have a taxonomy table from MetaPhlAn4, and I used q2-sapienns to create a feature table (relative frequency) that I can use in QIIME2 for analysis. I want to work with count data, and multiply all the relative abundances by a constant (ex: 100,000), ie total sum scaling. Is there a way to do this within the q2-sapienns plugin, or another QIIME2 plugin?

If I do total sums scaling directly to the mpa4 taxonomy file, it cannot be uploaded via q2-sapienns because it's not in the correct format.

Thanks for your help!

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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [July 20, 2023, 11:03pm UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138/2 "2023-07-20T23:03:31Z")

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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [July 20, 2023, 11:06pm UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138/3 "2023-07-20T23:06:49Z")

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Hi @em_joyce,  
You can do this using `qiime sapienns frequency --p-target-freq 100000`.

And I just want to point out this note from the [q2-sapienns](https://github.com/gregcaporaso/q2-sapienns) README.md file:

**q2-sapienns is comprehensively unit tested, but hasn't been field-tested much yet. If you notice any issues, please post to [the issue tracker](https://github.com/gregcaporaso/q2-sapienns/issues).**

Good luck!

Greg

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### Author: ![gregcaporaso](https://forum.qiime2.org/user_avatar/forum.qiime2.org/gregcaporaso/32/17769_2.png) [@gregcaporaso](https://forum.qiime2.org/u/gregcaporaso)
#### Post date: [July 20, 2023, 11:06pm UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138/4 "2023-07-20T23:06:54Z")

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [August 21, 2023, 5:07am UTC](https://forum.qiime2.org/t/q2-sapienns-total-sum-scaling/27138/5 "2023-08-21T05:07:02Z")

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