# q2-mOTUs beta release ๐
**URL:** https://forum.qiime2.org/t/q2-motus-beta-release/25296
**Category:** Plugins
**Tags:** taxonomy, metagenomics
**Created:** [January 24, 2023, 2:30pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296 "2023-01-24T14:30:59Z")
**Posts on this page:** 5
**Page:** 1
### Author:  [@crusher083](https://forum.qiime2.org/u/crusher083)
#### Post date: [January 24, 2023, 2:30pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296/1 "2023-01-24T14:30:59Z")
We are proud to announce **beta release of the q2-mOTUs** plugin ๐ ๐ ๐
**mOTU** is a tool for taxonomical profiling of metagenomic data extensively validated during [CAMI Challenge](https://www.nature.com/articles/s41592-022-01431-4).
# Principal concept
**mOTU** is an attempt to build a taxonomy utilizing genomic information about organisms formalized with the help of differences in 40 universal gene markers (40 MGs) sequences. The basic unit of the taxonomy is an **mOTU** , and thus it is used in the output. Detailed description of the approach can be found in the [paper](https://www.nature.com/articles/s41467-019-08844-4).
# Installation
The installation steps are described in our GitHub repo: [GitHub - motu-tool/q2-mOTUs](https://github.com/motu-tool/q2-mOTUs)
# Tutorial
There are two ways to use `mOTU-tool` within `QIIME 2`
## 1. Direct profiling of metagenomic samples
```auto
qiime motus profile \
--i-samples artifacts/study-seqs.qza \
--p-threads 8 \
--o-table artifacts/motu-table.qza \
--o-taxonomy artifacts/motu-taxonomy.qza
```
## 2. Import of precomputed `mOTUs` tables
**Attention** : precomupted mOTU table should be generated from full taxonomy (`-q` flag) and counts (`-c` flag) profiles.
```auto
qiime motus import-table \
--i-motus-table $TMPDIR/merged.motus \
--o-table artifacts/motu-table.qza \
--o-taxonomy artifacts/motu-taxonomy.qza
```
# Compatibility with other QIIME 2 tools
## Core QIIME 2
We can make a summary of the `FeatureTable[Frequency]` artifact `motu-table.qza`.
```auto
qiime feature-table summarize \
--i-table artifacts/motu-table.qza \
--o-visualization visualizations/motu-table-summary.qzv
```
[motu-table-summary.qzv](https://view.qiime2.org/visualization/?type=html&src=https%3A%2F%2Fdl.dropbox.com%2Fs%2Fvsedg96hb6uayjw%2Fmotu-table-summary.qzv%3Fdl%3D1)
## Other plugins
We can produce a beautiful Krona plot using `q2-krona`:
```auto
qiime krona collapse-and-plot \
--i-table artifacts/motu-table-feces.qza \
--i-taxonomy artifacts/motu-taxonomy.qza \
--o-krona-plot visualizations/motu-krona-feces.qzv
```
[motu-krona-feces.qzv](https://view.qiime2.org/visualization/?type=html&src=https%3A%2F%2Fdl.dropbox.com%2Fs%2F51kurmw326jxjie%2Fmotu-krona-feces.qzv%3Fdl%3D1)
# Extended tutorial
Extended version of this tutorial can be found in GitHub repo: [q2-mOTUs/tutorial at main ยท motu-tool/q2-mOTUs ยท GitHub](https://github.com/motu-tool/q2-mOTUs/tree/main/tutorial)
# Issue reporting
We encourage users to report possible problems either through QIIME2 Forum or [GitHub issue section](https://github.com/motu-tool/q2-mOTUs/issues). Me and @hjruscheweyh will provide the support.
# Citation
If you use the tool, please cite:
> Ruscheweyh, H.-J., Milanese, A., Paoli, L., Karcher, N., Clayssen, Q., Keller, M. I., โฆ Sunagawa, S. (2022). Cultivation-independent genomes greatly expand taxonomic-profiling capabilities of mOTUs across various environments. _Microbiome_, _10_ (1). doi:10.1186/s40168-022-01410-z
Yours sincerely
Valentyn Bezshapkin
on behalf of [Sunagawa Lab](https://micro.biol.ethz.ch/research/sunagawa.html)
---
### Author:  [@lizgehret](https://forum.qiime2.org/u/lizgehret)
#### Post date: [January 24, 2023, 5:31pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296/2 "2023-01-24T17:31:02Z")
Very exciting, thanks for all of your hard work @crusher083! ๐ฅณ
It's on my to-do list to look over this plugin and play around with it - I'll hopefully get to this in the next couple of weeks! Really looking forward to it!
---
### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 22, 2023, 2:46pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296/3 "2023-03-22T14:46:04Z")
3 off-topic replies have been split into a new topic: [installing q2-motus](https://forum.qiime2.org/t/installing-q2-motus/25870)
Please keep replies on-topic in the future.
---
### Author:  [@azithro13](https://forum.qiime2.org/u/azithro13)
#### Post date: [March 22, 2023, 12:28pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296/4 "2023-03-22T12:28:25Z")
Hi,
Bravo!!! we need simulation on youtube.
---
### Author:  [@crusher083](https://forum.qiime2.org/u/crusher083)
#### Post date: [March 22, 2023, 1:17pm UTC](https://forum.qiime2.org/t/q2-motus-beta-release/25296/5 "2023-03-22T13:17:48Z")
Thanks for your comment! I think it's possible to do, I'll update the topic when it's done.