# Q2-itsxpress param help

**URL:** https://forum.qiime2.org/t/q2-itsxpress-param-help/7935
**Category:** Library Support
**Created:** [January 22, 2019, 12:04pm UTC](https://forum.qiime2.org/t/q2-itsxpress-param-help/7935 "2019-01-22T12:04:51Z")
**Posts on this page:** 1
**Showing post:** 4

<div class="post-metadata">

### Author: ![MiriamGorostidi](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/m/6de8d8/32.png) [@MiriamGorostidi](https://forum.qiime2.org/u/MiriamGorostidi)
#### Post date: [February 14, 2022, 12:55pm UTC](https://forum.qiime2.org/t/q2-itsxpress-param-help/7935/4 "2022-02-14T12:55:32Z")

</div>

Hello @Adam_Rivers !

I created a topic in the forum last week ([Rarefying when having multiple runs - #16 by llenzi](https://forum.qiime2.org/t/rarefying-when-having-multiple-runs/22087/16) ) and they have recommended me to use your ITSxpress plugin.

I have had a glance at one of the first posts about the plugin ([Q2-ITSxpress: A tutorial on a QIIME 2 plugin to trim ITS sequences](https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780)) and I have a doubt before I use it.

The people that made the amplification of the samples decided to combine both ITS1 and ITS2 regions, so, how can I specified this in the --p-region parameter of the following command?

```auto
qiime itsxpress trim-pair-output-unmerged\
  --i-per-sample-sequences sequences.qza \
  --p-region ITS1 \
  --p-taxa F \
  --p-cluster-id 1.0 \
  --p-threads 2 \
  --o-trimmed trimmed_exact.qza

```

Thank you so much in advanced 🙂

---

_[View the full topic](https://forum.qiime2.org/t/q2-itsxpress-param-help/7935)._